凈化(hua)工(gong)(gong)作(zuo)臺是一種局部層流裝置,能(neng)在(zai)局部形(xing)成高(gao)潔度的工(gong)(gong)作(zuo)環境。它由工(gong)(gong)作(zuo)臺、過濾(lv)器、風機、靜壓箱和(he)支撐體等(deng)組成,采用過濾(lv)空(kong)氣(qi)使工(gong)(gong)作(zuo)臺操(cao)作(zuo)區達到凈化(hua)除菌(jun)(jun)的目的。室內空(kong)氣(qi)經(jing)預過濾(lv)器和(he)高(gao)效過濾(lv)除塵(chen)(chen)后(hou)以垂直(zhi)或水平層流狀態通(tong)過工(gong)(gong)作(zuo)臺的操(cao)作(zuo)區,由于空(kong)氣(qi)沒(mei)有渦流,所以,任(ren)何一點灰塵(chen)(chen)或附著在(zai)灰塵(chen)(chen)上的雜菌(jun)(jun)都能(neng)被排除,不易向(xiang)別(bie)處擴(kuo)散(san)和(he)轉移(yi)。因此,可使操(cao)作(zuo)區保持無(wu)菌(jun)(jun)狀態。
與無茵實操室和疫苗接種箱十分,實用清潔運行臺還具有運行生活條件好、實操便于、無茵實操效率靠得住、無殺菌消毒解毒劑對人身體威害、占綠地面積小且可中國電信等的優勢。只要擺到無茵實操室內吊頂實用,無茵實操效率更加好。其缺欠是單價貴,預進行濾芯過濾水器和優質進行濾芯過濾水器還必須 限期洗滌和換新。 高壓力水蒸氣滅菌處理鍋高壓蒸(zheng)(zheng)汽(qi)滅菌鍋(guo)是一(yi)個(ge)密(mi)閉的(de)(de)、可(ke)以耐受(shou)一(yi)定(ding)壓力(li)的(de)(de)雙層(ceng)金(jin)屬鍋(guo)。鍋(guo)底或夾層(ceng)內(nei)(nei)盛水(shui),當水(shui)在鍋(guo)內(nei)(nei)沸騰(teng)時由于蒸(zheng)(zheng)汽(qi)不能逸出,使(shi)鍋(guo)內(nei)(nei)壓力(li)逐漸升高,水(shui)的(de)(de)沸點和溫度(du)可(ke)隨之升高,從而達到高溫滅菌的(de)(de)目的(de)(de)。一(yi)般在0.11MPa的(de)(de)壓力(li)下(xia),121℃滅菌20~30min,包括(kuo)芽孢在內(nei)(nei)的(de)(de)所有(you)微生物均可(ke)被殺死。如果滅菌物品(pin)體積較大,蒸(zheng)(zheng)汽(qi)穿透困難(nan),可(ke)以適當提高蒸(zheng)(zheng)汽(qi)壓力(li)或延長滅菌時間。
進行高壓低壓低壓進行高壓低壓過熱蒸汽消毒鍋有離心式、臥式、手攜式式等各類種型,在分子生物學工程學檢測室,相對而言最常用的是手攜式式和臥式進行高壓低壓低壓過熱蒸汽進行高壓低壓過熱蒸汽消毒鍋。和過熱蒸汽進行高壓低壓過熱蒸汽消毒鍋比起,進行高壓低壓低壓進行高壓低壓過熱蒸汽消毒鍋的不足之處是進行高壓低壓過熱蒸汽消毒必備的時長短、省下染料、進行高壓低壓過熱蒸汽消毒撤底等。其不足之處是價錢珍貴,進行高壓低壓過熱蒸汽消毒體積較小。 培養箱培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)箱(xiang)(xiang)(xiang)是(shi)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)微(wei)(wei)生(sheng)物的(de)(de)專用(yong)(yong)設備(bei)。制熱式培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)箱(xiang)(xiang)(xiang)是(shi)由電爐絲和溫(wen)(wen)(wen)(wen)度(du)(du)控制儀合成的(de)(de)固(gu)定體積(ji)的(de)(de)恒(heng)溫(wen)(wen)(wen)(wen)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)裝(zhuang)置,大(da)小規格(ge)不(bu)一(yi)。微(wei)(wei)生(sheng)物實驗室常用(yong)(yong)的(de)(de)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)箱(xiang)(xiang)(xiang)工(gong)作容積(ji)有(you)450×450×350mm3或(huo)650×500×500mm3,適用(yong)(yong)于室溫(wen)(wen)(wen)(wen)至60℃之間(jian)的(de)(de)各類微(wei)(wei)生(sheng)物培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)。目前,隨著(zhu)科學水平的(de)(de)發(fa)展,培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)箱(xiang)(xiang)(xiang)設備(bei)的(de)(de)完善程度(du)(du)和價格(ge)有(you)很大(da)差別。有(you)各種結構(gou)合理、功能齊全的(de)(de)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)箱(xiang)(xiang)(xiang),如恒(heng)溫(wen)(wen)(wen)(wen)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)箱(xiang)(xiang)(xiang)、恒(heng)溫(wen)(wen)(wen)(wen)恒(heng)濕(shi)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)箱(xiang)(xiang)(xiang)、低溫(wen)(wen)(wen)(wen)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)箱(xiang)(xiang)(xiang)、微(wei)(wei)生(sheng)物多用(yong)(yong)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)箱(xiang)(xiang)(xiang)和二(er)氧化(hua)碳培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)箱(xiang)(xiang)(xiang)等(deng)。有(you)的(de)(de)用(yong)(yong)計算機控制,可選擇多條時間(jian)線變換溫(wen)(wen)(wen)(wen)差,從而克服(fu)了環境溫(wen)(wen)(wen)(wen)度(du)(du)的(de)(de)影(ying)響,一(yi)年四季均能達到(dao)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)要求的(de)(de)溫(wen)(wen)(wen)(wen)度(du)(du)。
微生物多用(yong)培養箱是集(ji)加熱、制(zhi)冷和振蕩于一體(ti)的微生物液(ye)體(ti)發酵裝置(zhi)。工(gong)作(zuo)室的溫(wen)度在(zai)15~50℃范圍內(nei)任意選(xuan)定,選(xuan)定后經溫(wen)控(kong)儀自動控(kong)制(zhi),保持工(gong)作(zuo)室內(nei)恒溫(wen)。同時設有可控(kong)硅(gui)調速(su)系統,振蕩機轉(zhuan)速(su)可在(zai)1~220rpm范圍內(nei)任意調控(kong)。
干躁箱干(gan)(gan)燥(zao)(zao)箱(xiang)(xiang)是用(yong)(yong)于除去潮濕物(wu)料(liao)內及器(qi)皿內外(wai)水分或(huo)其它(ta)揮發(fa)性溶液的(de)設(she)備。類型很多(duo),有(you)(you)箱(xiang)(xiang)式(shi)、滾筒式(shi)、套間式(shi)、回轉(zhuan)式(shi)等。微生物(wu)學實驗室多(duo)用(yong)(yong)箱(xiang)(xiang)式(shi)干(gan)(gan)燥(zao)(zao)箱(xiang)(xiang),大小規格不一。工作(zuo)室內配(pei)有(you)(you)可(ke)活動的(de)鐵絲網(wang)板,便(bian)于放置被干(gan)(gan)燥(zao)(zao)的(de)物(wu)品。制(zhi)熱升(sheng)溫(wen)(wen)式(shi)干(gan)(gan)燥(zao)(zao)箱(xiang)(xiang)也是有(you)(you)電爐絲和溫(wen)(wen)度控制(zhi)儀(yi)組成,可(ke)調節溫(wen)(wen)度從室溫(wen)(wen)至300℃任意選擇。有(you)(you)的(de)干(gan)(gan)燥(zao)(zao)箱(xiang)(xiang)采用(yong)(yong)導(dao)電溫(wen)(wen)度計為(wei)敏感元件,配(pei)合晶體(ti)管和繼(ji)電器(qi)組成自動控制(zhi)系統,克服了(le)金屬管型熱膨(peng)脹控制(zhi)的(de)缺(que)點。此外(wai),還有(you)(you)真空干(gan)(gan)燥(zao)(zao)箱(xiang)(xiang)(配(pei)有(you)(you)真空泵和氣壓(ya)表(biao)),可(ke)在常壓(ya)或(huo)減壓(ya)下操作(zuo)。
搖床
搖(yao)床又稱(cheng)搖(yao)瓶(ping)機,它是(shi)培(pei)養好氣(qi)性微生物的小型(xing)試驗設備(bei)或作為(wei)(wei)種(zhong)子(zi)擴(kuo)大培(pei)養之用(yong),常(chang)用(yong)的搖(yao)床有往復式(shi)和旋轉(zhuan)(zhuan)式(shi)兩種(zhong)。往復式(shi)搖(yao)床的往復頻率一般(ban)在80~140次(ci)(ci)/min,沖(chong)程一般(ban)為(wei)(wei)5~14cm,如頻率過快、沖(chong)程過大或瓶(ping)內液體裝(zhuang)量過多,在搖(yao)動(dong)時(shi)液體會濺到包扎瓶(ping)口的紗布(bu)或棉塞上(shang),導致雜(za)菌污染(ran),特別是(shi)啟動(dong)時(shi)更容(rong)易發生這種(zhong)情況(kuang)。旋轉(zhuan)(zhuan)式(shi)搖(yao)床的偏心距一般(ban)在3~6cm之間,旋轉(zhuan)(zhuan)次(ci)(ci)數為(wei)(wei)60~300rpm。
放在搖(yao)床上的(de)(de)(de)培養瓶(一(yi)般為三(san)角瓶)中(zhong)的(de)(de)(de)發酵(jiao)液所(suo)需要的(de)(de)(de)氧是由空氣經瓶口包扎(zha)的(de)(de)(de)紗(sha)布(一(yi)般8層)或(huo)棉塞(sai)通(tong)入的(de)(de)(de),所(suo)以氧的(de)(de)(de)傳遞與瓶口的(de)(de)(de)大小、瓶口的(de)(de)(de)幾何(he)形(xing)狀、棉塞(sai)或(huo)紗(sha)布的(de)(de)(de)厚(hou)度和密度有(you)關。在通(tong)常情況下,搖(yao)瓶的(de)(de)(de)氧吸(xi)收系數(shu)取決于(yu)搖(yao)床的(de)(de)(de)特性和三(san)角瓶的(de)(de)(de)裝樣量。
往返式搖床是借助曲柄作用助推搖床作往返動作,主機為鐵質或廚衛電器的長方框子,有顆層至二三層包裝箱,包裝箱上都有小圓孔備放激發出來瓶,孔中突出另一個三邊形形橡膠,主要用于進行固定激發出來瓶并以減少瓶的振蕩,傳送構造一樣 按照中級這根牛三角帶輪減速運動,互換可控硅調壓這根牛三角帶輪可改善往返率。反力距輪上都有各不相同的反力距孔,事先的調節反力距距。往返式搖床的率和反力距距的長寬對氧的釋放有凸顯的印象。 回轉式搖床是采用回轉的反力軸使餐盤搖擺,餐盤一 層或二屋,可以用不銹不銹鋼裝飾管鋼板、鋁塑板或木質板研制。在多個反力軸上配有螺栓螺母調節節左右,使餐盤始終維持級別。在這種搖床設計非常復雜,造貴點。其優點有哪些是氧的傳達著很好、輸出功率所耗小、培養出基不要濺到瓶口的紗布繃帶上。 顯微鏡觀察 產氣莢膜梭菌學個人用戶很小,需求通過光學體視顯微鏡才會觀查知曉它是的個人用戶價值形式和細胞核格局。因而,在產氣莢膜梭菌學學的數據來研究方案中,光學體視顯微鏡就成為不要丟失的工具軟件。顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)的(de)(de)種類很多,根據(ju)其(qi)結構,可以分(fen)為(wei)(wei)光(guang)(guang)學(xue)(xue)顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)和(he)非光(guang)(guang)學(xue)(xue)顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)兩大(da)類。光(guang)(guang)學(xue)(xue)顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)又可分(fen)為(wei)(wei)單(dan)式顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)和(he)復(fu)式顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)。最(zui)(zui)簡單(dan)的(de)(de)單(dan)式顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)即放大(da)鏡(jing)(jing)(jing)(jing)(jing)(放大(da)倍數常(chang)在(zai)10倍左(zuo)右(you)),構造復(fu)雜的(de)(de)單(dan)式顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)為(wei)(wei)解剖顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)(放大(da)倍數在(zai)200左(zuo)右(you))。在(zai)微(wei)(wei)(wei)(wei)(wei)生物學(xue)(xue)的(de)(de)研究(jiu)中,主要(yao)是復(fu)式顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)。其(qi)中以普通光(guang)(guang)學(xue)(xue)顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)(明視野顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing))最(zui)(zui)為(wei)(wei)常(chang)用(yong)。此(ci)外,還有暗視野顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)、相差顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)、熒光(guang)(guang)顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)、偏光(guang)(guang)顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)、紫外光(guang)(guang)顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)和(he)倒置(zhi)顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)等。非光(guang)(guang)學(xue)(xue)顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)為(wei)(wei)電子顯(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)(jing)。
注射箱接(jie)種(zhong)(zhong)箱(xiang)(xiang)(xiang)分(fen)(fen)為(wei)(wei)固體菌種(zhong)(zhong)接(jie)種(zhong)(zhong)箱(xiang)(xiang)(xiang)和液體菌種(zhong)(zhong)接(jie)種(zhong)(zhong)箱(xiang)(xiang)(xiang)兩種(zhong)(zhong)。固體菌種(zhong)(zhong)接(jie)種(zhong)(zhong)箱(xiang)(xiang)(xiang)是一個(ge)用木(mu)(mu)料和玻(bo)(bo)璃(li)制成(cheng)或由(you)有機玻(bo)(bo)璃(li)焊(han)接(jie)而成(cheng)的(de)密(mi)閉(bi)小(xiao)箱(xiang)(xiang)(xiang)。又分(fen)(fen)為(wei)(wei)雙(shuang)人和單人操作箱(xiang)(xiang)(xiang)。箱(xiang)(xiang)(xiang)體可大可小(xiao),一般箱(xiang)(xiang)(xiang)體長約143cm,寬86cm,總高(gao)154cm,支(zhi)(zhi)架76cm。箱(xiang)(xiang)(xiang)的(de)上部(bu)(bu)左(zuo)右兩側各(ge)裝(zhuang)有兩扇能啟閉(bi)的(de)玻(bo)(bo)璃(li)推拉門,方(fang)便(bian)菌種(zhong)(zhong)進(jin)出。窗的(de)下部(bu)(bu)分(fen)(fen)別設有兩個(ge)直徑約13cm的(de)圓洞(dong)(dong),兩洞(dong)(dong)的(de)中心距離為(wei)(wei)52cm(同肩寬),洞(dong)(dong)口裝(zhuang)有帶松(song)緊帶的(de)袖(xiu)套,以防雙(shuang)手(shou)在箱(xiang)(xiang)(xiang)內操作時,外界空氣進(jin)入箱(xiang)(xiang)(xiang)內造成(cheng)污(wu)染。操作時兩人相對而坐,雙(shuang)手(shou)通(tong)過袖(xiu)套伸入箱(xiang)(xiang)(xiang)內。箱(xiang)(xiang)(xiang)兩側最好也裝(zhuang)上玻(bo)(bo)璃(li),箱(xiang)(xiang)(xiang)頂(ding)部(bu)(bu)為(wei)(wei)木(mu)(mu)板或玻(bo)(bo)璃(li)。箱(xiang)(xiang)(xiang)內頂(ding)部(bu)(bu)裝(zhuang)有紫外線殺菌燈(deng)和照明用日光(guang)燈(deng)各(ge)一支(zhi)(zhi)。箱(xiang)(xiang)(xiang)體安裝(zhuang)木(mu)(mu)板或玻(bo)(bo)璃(li)均(jun)可,但(dan)要注意密(mi)封。
液(ye)體(ti)菌種(zhong)(zhong)(zhong)接種(zhong)(zhong)(zhong)箱(xiang)(xiang)是專(zhuan)為移(yi)接液(ye)體(ti)菌種(zhong)(zhong)(zhong)而設(she)計的(de)(de)。比固體(ti)菌種(zhong)(zhong)(zhong)箱(xiang)(xiang)窄長(chang),單(dan)側(ce)兩人操作。內(nei)設(she)軌(gui)道和紫外線燈(deng),箱(xiang)(xiang)兩端(duan)開(kai)有高(gao)25cm,寬(kuan)10cm的(de)(de)長(chang)方(fang)形出(chu)(chu)(chu)口(kou)(kou),方(fang)便菌種(zhong)(zhong)(zhong)進(jin)(jin)(jin)出(chu)(chu)(chu),洞口(kou)(kou)設(she)有小推(tui)門。進(jin)(jin)(jin)出(chu)(chu)(chu)口(kou)(kou)下處設(she)蒸汽源,接種(zhong)(zhong)(zhong)時用蒸汽封(feng)住(zhu)進(jin)(jin)(jin)出(chu)(chu)(chu)口(kou)(kou),以防(fang)雜(za)菌進(jin)(jin)(jin)入箱(xiang)(xiang)內(nei)。箱(xiang)(xiang)背面設(she)有液(ye)體(ti)菌種(zhong)(zhong)(zhong)移(yi)接管能(neng)進(jin)(jin)(jin)入的(de)(de)小孔(kong)。
接(jie)種(zhong)(zhong)箱(xiang)(xiang)(xiang)滅菌時(shi),用(yong)紫外(wai)線照射30min。如果沒有紫外(wai)線燈,可用(yong)甲醛和(he)高(gao)錳酸鉀(甲醛10~14mL/m3+高(gao)錳酸鉀5~7g/m3空(kong)間)熏蒸30min以(yi)上。使用(yong)時(shi),先將所需物品和(he)工具放入(ru)接(jie)種(zhong)(zhong)箱(xiang)(xiang)(xiang)內,然后進(jin)(jin)行藥劑熏蒸和(he)紫外(wai)線滅菌,再按無菌操作進(jin)(jin)行接(jie)種(zhong)(zhong)。接(jie)種(zhong)(zhong)箱(xiang)(xiang)(xiang)的(de)結(jie)構簡(jian)單,造價低(di)廉,易消(xiao)毒滅菌,操作方便,而且人在箱(xiang)(xiang)(xiang)外(wai)操作,氣溫較高(gao)時(shi)也能作業。缺點是進(jin)(jin)出培(pei)養(yang)基費(fei)(fei)工費(fei)(fei)時(shi),每次接(jie)種(zhong)(zhong)前都(dou)需要進(jin)(jin)行滅菌。
冷柜 微生物實驗室的冰箱主要有兩種:普通冰箱和低溫冷凍冰箱。普通冰箱一般都具有兩個柜子,即鮮藏柜和冷藏柜,溫度分別為4℃和-20℃;低溫冷凍冰箱溫度一般控制在-40~-80℃。它們都可以用于微生物菌種保藏。鮮藏柜常用于保存斜面菌種,保藏時間在3個月左右。超過3個月,斜面就會變干,因此需要轉接菌種。如果要長時間保存菌種,則需要經過處理后,貯藏于普通冰箱的冷藏柜或低溫冷凍冰箱中,它們的保藏時間較長,一般都在1年以上。